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Skin Reactions Skin reactions that may occur following a vitamin B12 shot include flushing of the skin, (especially the face), as well as hives, rashes, and itching

Finding the optimal buffer composition: high salt concentration (for example, optimal purity can typically be achieved with buffer containing 300 mM NaCl at pH 8.0 for some proteins), pH, as well as addition of the right type of detergents at the right concentration (this is particularly important for membrane proteins or the proteins prone to aggregate)