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Confirms no viable microorganisms are present - going beyond sterile filtration alone

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The inhibition of ABTS + formation by antioxidants in the samples were expressed as Trolox equivalents, determined at 740 mm using a calibration curve plotted with different amounts of Trolox (Sigma Aldrich) ( Glutathione (GSH) Levels The level of GSH was determined in samples of serum and peritoneal lavage fluid of septic mice at 12 and 24 h post CLP induction using Ellman's reagent ( In vitro Studies Peripheral Blood Mononuclear Cells (PBMC) Isolation and in vitro Stimulation PBMC of healthy volunteers who were abstainers of alcohol and tobacco (both sexes, ages 20 to 45 years) were isolated from blood using Ficoll (Sigma-Aldrich)