Editing efficiency was determined on bulk-edited cell populations by targeted PCR of the Cas9-targeted region and secondary PCR using overhang sequences (Table S1), followed by Illumina MiSeq, as previously described (Aubrey et al, 2015)
[DOI] [PubMed] [Google Scholar] 2.Williams HE
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Considering that both carnitine and synbiotics are perceived to induce beneficial effects on glycemic indices (10, 77), hence, significant intergroup differences in glycemic parameters might have been mediated through the plausible complementary or cumulative effects of L-carnitine + synbiotic co-administration, which led to a greater amelioration in glycemic indices in the co-supplemented arm, or conceivably, could be attributed to the greater weight reduction in the co-supplemented group (78)