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Rayman, 2012)

It has been reported that the N-terminus of DAT is essential for protein kinase C-mediated phosphorylation on serine residues [67], and the first 22 amino acids from the N-terminus of DAT eliminate phosphorylation of DAT in response to PKC activation [44]

Quantitative real-time PCR (qRT-PCR) analysis Total RNA was isolated from cortical samples by using RNAiso plus (Takara, Shiga, Japan) according to the manufacturers instructions