glp-1 agonists vs sglt2 inhibitors Mechanisms underlying favorable effects of GLP-1R and GLP-1 receptor agonists vs. SGLT-2

7-Dichlorofluorescin Diacetate (DCFDA) Assay for Production of Reactive Oxygen Species Cells were incubated in 10 M DCFDA (Sigma) in PBS at 37C for 30 min as previously described (Chiu et al., Quantification of 8-Hydroxy-2-Deoxyguanosine (8-OH-dG) as a Marker for Oxidative DNA Damage We used 8-Hydroxy-2-Deoxyguanosine (8-OH-dG) as a biomarker for oxidative DNA on brain cells exposed to different treatments using the commercially available HT 8-OH-dG ELISA II kit (R&D Systems) as guided by the manufacturers instructions

They identified the big 40 Dalton protein, BPC

/ / / / - L-Carnitine Solgar 300 " Solgar GLA 300 mg 60 252 .04 4.20 -1 400 - 30 27 .74 0.92 -1 D 60 66 .11 1.10 -1 500 " 20 39 .87 1.99 -1 prenatal 30 36 .12 1.20 -1 30 " Dr

15,000 tests per month verify ingredient potency and purity.^ In an industry where quality can vary widely, testing isn't just a step in the process