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doi: 10.1016/S0140-6736(08)60104-X 16

According to clinical resources such as the Mayo Clinic, while B12 is essential for nerve and red blood cell health, theres no strong evidence that injections improve energy or aid weight loss in people without a documented deficiency

This comes from animal studies and was not replicated during placebo-controlled clinical trials

The reaction mixture was then centrifuged (4 C, 10,000 rpm, 15 min), filtered through a 0.45 m filter, and subjected to a second Ni-NTA agarose purification step