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To quantify mRNA, following cDNA synthesis, the quantitative RT-PCR (qRT-PCR) analysis was carried out on an Applied Rotor-Gene 3000 with the following conditions: GAPDH and target genes were amplified for 40 cycles at 94C for 30 seconds, 63C for 60 seconds, and 72C for 90 seconds

Our intramuscular vitamin injections deliver concentrated doses of essential nutrients like B12, biotin, glutathione, vitamin D, and lipotropic compounds directly into the muscle for rapid absorption

[DOI] [PubMed] [Google Scholar] Su LJ, Zhang JH, Gomez H, Murugan R, Hong X, Xu D, Jiang F, Peng ZY