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a-c , Difference in abundance in the extracellular cysteinylglycine in medium (a) and relative abundance of intracellular cysteinylglycine (b) and intracellular cysteine (c) for HCC-1806 cells grown in the indicated conditions and time points (n = 3 technical replicates representative from 2 independent experiments, except for c at 24 h, in which n = 2 technical replicates from 2 independent experiments)

In peptide research, variability can enter through inconsistent reconstitution assumptions, adsorption effects at low working concentrations, uncontrolled temperature excursions during preparation, and untracked freeze thaw events that shift effective delivered exposure