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This article explores these intricacies in an explanation on how using marijuana impacts antidepressants

20 A major factor in these results is that the mechanism of action of exenatide in targeting PD is unclear

To remove the ligands in the solution, the pellets were washed with PBS, centrifuged at 10,000 g for 30 min at 4 C, and re-suspended in the sonication buffer containing 50 mM HEPES (pH 7.4), 25 mM NaCl, 1 mM EDTA, 1 mM MgCl 2 , and 0.5% Triton X-100