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In the context of cancer treatment, while interventions such as chemotherapy and radiotherapy have markedly enhanced patient survival rates, their gonadotoxic effects can lead to ovarian failure and infertility, particularly affecting women of reproductive age and pre-pubertal girls (2)

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Fusion constructs ROGFP2 WT -PFGRX WT , ROGFP2 WT -PFGRX C88S , and ROGFP2 WT -PFGRX C32S were PCR-amplified with Phusion DNA polymerase using the corresponding p416TEF plasmids as a template 35 and subcloned into the Kpn I and Avr II restriction sites of pET45b in strain XL1-Blue

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