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The authors show that the drastic growth arrest of the sir1 mutant is partially rescued when glutathione deficiency is introduced via the cad2 mutation, which decreases glutathione levels to 30% to 40% of wild-type levels (Cobbett et al., 1998), or pharmacologically through treatment with the GCL inhibitor buthionine sulfoximine

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Notably, in the ligand-free GIPR, the TM6ECL3 juncture is sandwiched by the TM5ECL2 juncture and the extracellular portion of TM7, weaving an intensive interaction network by a hydrogen bond between Q220 3.33b and R190 2.67b , and several hydrophobic contacts (R300 5.40b V355 6.54b /V356 6.55b , W274 4.60b Q224 3.37b , and W287 ECL2 L194 2.71b /V360 6.59b ) (Fig