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0.05 g fresh mice lung were homogenized by with 1 mL PBS, and centrifuged at 1,600 g for 10 min at 4 C

220 While IRE1 and ATF6 upregulate HRD1, prolonged ER stress downregulates bifunctional apoptosis regulator (BAR), which is known to mediate proteasomal degradation of the ER-resident IRE1 inhibitor protein Bax inhibitor-1 (BI-1), which controls IRE1 signaling

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