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This is determined by measuring the luminol chemiluminescence intensity of emission induced by thermolysis of 2,2-azobis (2-amidinopropane) hydrochloride (AAPH) as free radical source

As a cysteine prodrug, N-acetylcysteine (NAC) is capable of rapidly replenishing intracellular cysteine pools for GSH biosynthesis, which also enhances the activity of GPX4 (133)

doi:10.1016/0196-9781(86)90148-7 PMID: 3550877 8