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Studies have demonstrated that germ-free animals display cecal enlargement, reduced intestinal surface area, and gastrointestinal dysregulation ( 3.1 Intestinal mucosa As the innermost layer of the intestinal wall, the mucosa is divided into the small and large intestinal segments

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It incorporates key modifications, including 14E/17R mutations to stabilize an -helical segment via a salt bridge, multiple proline substitutions (25P, 28P, 29P) to reduce -sheet formation, and N-terminal C20 fatty diacid lipidation for reversible albumin binding (Kruse et al

Moreover, there is a scarcity of studies addressing these effects in bulls