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Arabidopsis vtc2-1 accumulates only 10 to 30% of the wildtype Asc level, which results from a mutation in the GDP-l-galactose phosphorylase 1 gene, the enzyme catalyzing an intermediate step in Asc synthesis (Mller-Moul et al

Total RNA extraction and complementary DNA library construction Groups of unfed adults (5 females and 5 males in each group) were collected, put into a precooled mortar and ground with liquid nitrogen into powder, then moved to a 1.5-ml EP tube for total messenger RNA (mRNA) extraction

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