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Immediate steps (first 0 to 30 minutes) Right after the shot, follow these straightforward steps: keep pressure light rather than squeezing, apply a cold pack for 10 to 15 minutes, and wear loose clothing over the area

For the final two syringes containing the cDNA-decorated capture gels, 1.6 mL of a 2 gel and enzyme cocktail was prepared by mixing gels and water with a final concentration of Optiprep (16% v/v), 0.5 mg/mL BSA, 10 VH forward primers each at 0.2 M, a single V reverse primer at 2 M, 2% (v/v) PVA and a cocktail of inner primers annealing to both the heavy and light chain where each primer is at a concentration of 7.6 nM and 3.8 nM respectively

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