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In brief, plated cells were incubated for 30 min at 37 C, 5% CO 2 in the staining solution (HBSS containing 1 g ml 1 Hoechst 33342 (62247, Invitrogen, Thermo Fisher Scientific) and either 5 M CellROXTM, 1 M FerroOrange or 1 M BODIPY 581/591 C11)

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JAMA Network Open 7(5):e2413172 Valentine WJ, Hoog M, Mody R, Belger M, Pollock R (2023) Long-term cost-effectiveness analysis of tirzepatide versus semaglutide 1.0 mg for the management of type 2 diabetes in the United States

Based on the available data, short-term effects related to MT-I and MT-II consist of the following [5, 18]: Nausea Facial flushing Fatigue Dizziness In addition, clinical studies on MT-II have reported several other specific side effects [19, 23]: Yawning Stretching Spontaneous erections Prolonged, painful erections (priapism) The administration of MT-I and MT-II can rarely cause reactions at the injection site, such as redness, swelling, and bleeding