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By elucidating the coordinated roles of these mechanisms, this research aims to provide novel insights into PCOS pathogenesis and demonstrate the potential of quercetin as a complementary treatment, particularly when combined with trace element supplementation, to improve reproductive and metabolic outcomes in PCOS
Atherosclerosis 237 , 208219 (2014)
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To charge RHEB with GTPS (Abcam) or GDP (Sigma-Aldrich), RHEB was first diluted in buffer (50 mM HEPES, pH 7.5, 150 mM NaCl, 1 mM TCEP, 5 mM EDTA) and then supplemented with GDP or GTPS at a 30-fold molar excess
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