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Sigma-Aldrich) were cultured in DMEM media (Gibco) supplemented with 15% FBS (Gibco) and 1% PSN (Gibco)

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Yeast cells were spread on YPD agar plates (YPD medium with 15 gL 1 agar) containing nourseothricin (200 g mL 1 ) and incubated at 30 C for 3 days

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