Ethics statement The animal study was approved by the experimental protocol was approved by the Institutional Animal Care and Use Committee of Jining Medical University (Approval No
In addition, previous studies have shown that ferroptosis and pyroptosis are also associated with granulosa cell death in polycystic ovarian syndrome (PCOS) and diminished ovarian reserve (DOR) ( Gpx4 ), Solute Carrier Family 7 Member 11 ( Slc7a11 ), Acyl-CoA Synthetase Long Chain Family Member 4 ( Acsl4 ), Lysophosphatidylcholine Acyltransferase 3 ( Lpcat3 ) , Apoptosis Inducing Factor Mitochondria Associated 2 ( Aifm2 ), and GTP Cyclohydrolase 1 ( Gch1 ), and the pyroptosis-related genes, including Caspase 1 ( Casp1 ), Caspase 11 ( Casp11 ) , Gasdermin D ( Gsdmd ), and Gasdermin E ( Gsdme ), were analyzed by qPCR
10.1038/mp.2013.114 163
For native IGF-1, binding to IGFBP-3 and ALS lengthens circulating half-life the ternary complex protects the peptide from rapid renal clearance and proteolysis, so bound native IGF-1 persists in blood for hours rather than the roughly 10 minutes reported for free IGF-1
