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The columns were washed with 15 column volumes of buffer 1 followed by the elution with 200 mM imidazole, 50 mM Na x H y PO 4 , 300 mM NaCl, pH 8.0 at 4 C

Unfortunately, veterinary application, trials, and research in dogs are still 510 years behind human medicine, but interest in the field is rapidly accelerating

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Annu Rev Med , 2004, 55: 395-417