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For experiments visualising axonal projections from brainstem PPG neurons PPG-YFP mice and PPG-Cre mice were crossed, and their YFP- and Cre-positive offspring ( n = 3) injected bilaterally with AAV2-Ef1a-DIO-tdTomato (0.25 l/injection

GLP-1R knockout mice (GLP-1R KO, Shanghai Model Organisms, based on C57BL/6N) were generated using CRISPR/Cas9 technology, which causes Glp1r gene protein reading frame shift and loss of function by using non-homologous recombination repair to introduce mutations

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While there are limitations, the promise of improved diabetes management, cardiovascular benefits, and new formulations places GLP-1 agonists at the forefront of diabetes care