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Li N, Sioutas C, Cho A, Schmitz D, Misra C, et al: Ultrafine particulate pollutants induce oxidative stress and mitochondrial damage

This in turn alters downstream testosterone, estradiol, and progesterone levels, which impact ovulation timing and sperm production

After 48 h, the cells were washed with PBS (Gibco) and incubated in the assay buffer (HBSS supplemented with Ca 2+ and Mg 2+ (Gibco), 10 mM HEPES (Gibco), 0.1% Pluronic F-68 (Gibco) and 500 M IBMX (Sigma), pH 7.4) with different concentrations of GLP-1 (0 M to 1 M) in the presence (100 nM and 1000 nM) or absence of Fab7F38 at 37 C for 30 min

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