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Prediction of membrane transport proteins and their substrate specificities using primary sequence information

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After stirring for 20 min at room temperature, 10 L of 10 mM NaBH 4 solution was added, and the mixture was vigorously stirred for 10 min at room temperature in the dark

The 3-D cell model was made by culturing cells (2 10 5 1.0 10 6 ) with high glucose DMEM supplemented with 30% bovine collagen solution overnight