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In the cellular context, these aberrant interactions can disrupt physiological processes by sequestering various proteins essential for normal cellular functions, resulting in diffused aggregates that may act as molecular triggers for LB nucleation
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Cycling conditions consisted of an initial denaturation step at 95 C for 3 min, followed by 40 cycles at 95 C for 30 s, 60 C for 30 s, and 72 C for 30 s, and then 72 C for 10 min
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