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Not intended for diagnosis, treatment, or prevention of diseases

coli cells (100 mL) were collected (6000 rpm, 4 C, 10 min) and resuspended in 5 mL of lysis buffer containing 8 M urea, 4 % (w/v) CHAPS, and 1 % (w/v) DTT and sonicated on ice for 90 cycles (a working period of 3 s in a 6 s interval for each cycle) at a power output of 200 W by an ultrasonic disruptor (JY92-II, Scientz Biotechnology Co., Ningbo, China)

Along with possible direct toxicity, elevated phenylalanine levels are thought to decrease the cerebrospinal fluid (CSF) concentrations of tyrosine and its downstream products like serotonin and dopamine

Since growth hormone release is regulated by the hypothalamus and pituitary gland, any influence on these neural systems might indirectly affect testosterone production