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An alternative interpretation for the first phase could be a rapid noncovalent interaction between PfGrx and GSSG in the vicinity of roGFP2, however, this seems less likely considering the smaller values for the structurally similar PfGrx mutants or, in particular, the rxYFP fusion constructs with ScGrx1 (Table 1)

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GPX4 is a prognostic predictor and has the potential to become an anticancer drug target for NSCLC treatment

Super Vision Two-Step Polymer Non-Biotin HRP Detection System for DAB (Boster, Wuhan, China) was used for secondary antibody staining according to the manufacturers instructions