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Mice were euthanized using gradual-fill CO inhalation in accordance with the AVMA Guidelines for the Euthanasia of Animals (2020), followed by cervical dislocation to ensure complete euthanasia before tissue collection

The NAD+/NADH ratio is a primary control point linking hundreds of metabolic reactions throughout the cell, and its dysregulation is implicated in a wide range of pathological states from metabolic syndrome and neurodegeneration to cardiovascular disease and accelerated cellular ageing