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Following dehydration in ascending concentrations of ethanol solutions (5 min washes in 50%, 70%, 100%, and 100% ethanol), slides were air-dried, and a hydrophobic barrier was created around the sections using a hydrophobic pen (Vector Labs)

Waring MJ, Arrowsmith J, Leach AR, Leeson PD, Mandrell S, Owen RM, et al

Fibromyalgia affects up to 3% of the general population and is a syndrome characterised by widespread pain, fatigue, sleep disturbances, and cognitive dysfunction, somatic symptoms, and psychiatric disorders [90]