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Oftentimes, when doing pull-down experiments, researchers will include a control experiment using GST alone instead of their GST-fusion protein

Clinics (2018) 73(suppl 1):e473s

Furthermore, a 2-year, double-blind, randomized, placebo-controlled therapeutic trial in 158 healthy siblings of hyperhomocysteinemic patients with premature atherothrombotic disease folic acid plus vitamin B 6 treatment was associated with a decreased occurrence of abnormal exercise electrocardiography tests, which is consistent with a decreased risk of atherosclerotic events (Vermeulen et al, 2000)

350,351 Loss-of-function mutations in Parkin (PARK2), an E3 ubiquitin ligase, underlie autosomal recessive Parkinsons disease