However, in type 2 diabetes mellitus patients, the neuronal assessment included peripheral vibration perception and temperature in both studies, but Ponirakis et al
Rotate between 4 quadrants each week: right upper, left upper, left lower, right lower
For Ki67 and p-ERK stainings, blocking and primary antibody dilution were performed using PB buffer composed of 0.05% milk powder (#T145.2, Carl Roth, Germany), 0.25% fish gelatin (#G7765, Sigma-Aldrich, USA), 0.5% Triton X-100 (#BP151-100, Fisher Bioreagents, Germany), 20 mM HEPES (pH 7.2, #9105.4, Carl Roth, Germany), and 0.9% NaCl (#3957.1, Carl Roth, Germany)
We also assess markers of inflammation, liver function, and oxidative stress to get a full picture of your internal environment