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A: Frequency depends on your individual health goals and your providers recommendation

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A 10% kidney homogenate was prepared, and SOD, GSH/GSSG, GSH, GSH-Px, and MDA activities were determined according to the instructions provided with the kit

The residue was reconstituted to 150 L by adding 150 L of 25 MeOH : 25 water : 50 acetonitrile containing 250 ng/mL of debrisoquine (DBQ) as internal standard for positive mode and 250 ng/mL of 4-nitrobenzoic acid as internal standard for negative mode