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ROS would thus have a double function , as actors of a deleterious oxidative stress , but also as signal molecules activating gene transduction and activation processes, regulating the normal processes of growth and development, but also presumed to participate in cellular defense (Figure 9)

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increase by ~50 mg every 12 weeks as tolerated

Cryo-EM methods Negative staining transmission electron microscopy The homogeneity of the protein before structural determination was assessed by negative staining electron microscopy