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During the incubation time, 5 L of 80mM glucose, 9 M oligomycin, and 1 M 2-deoxyglucose (for ECAR measurement) or 10M oligomycin, 9 M FCCP, 10 M Rotenone, 10 M antimycin A (for OCR measurement), were loaded in XF assay media into the injection ports in the XFe-96 sensor cartridge

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