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gossypii and yeasts, FMN is synthesized by riboflavin kinase encoded by the FMN1 gene, which could be the major rate-limiting step for FAD provided by the FAD1 gene product, FAD-synthase ( Riboflavin Biosynthesis Regulation A variety of inducers, effectors, inhibitors, and signal molecules affect metabolite overproduction in microorganisms that provide positive or negative regulation of enzyme catalyzing metabolic reactions, through regulatory genes responsible for feedback inhibition (transcription and translation levels) or allosteric effects on some enzymes (posttranslational level) ( RIB3, RIB4 , and RIB5 in A