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Rats had ad libitum access to water and SD (Picolab, rodent diet 5053) until 10 months of age in which they were divided into weight-matched groups within each sex, and fed a SD (Picolab, rodent diet 5053) or WD (Research Diets (RD) Western Diet, D12079B, Research Diets Inc., New Brunswick, NJ, USA) for the following 8 weeks of the experiment as described below and demonstrated in Figure 1A

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