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Prevents Weight Gain: Unlike other diabetes medications, Metformin is not associated with weight gain

Proteins were visualized using the Supersignal West Pico Chemiluminescent Substrate system (Pierce, Fenton, MO) and molecular weights determined by comparison to protein ladders MDCK cells were grown to 100% confluency on the coverslips in six-well plates and washed twice with PBS

The homogenate was then centrifuged at 4000 rpm/5 minutes at 4 C in a cooling centrifuge then 0.5 ml of the supernatant was added to 0.5 ml of trichloro-acetic acid 10%

The commenters believe that the deletion of this code may negatively impact patient care, since many patients do not report every relevant symptom or SDOH risk factor without first being prompted by a physician