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The toxicity of free iron is related to its ability to rapidly generate the highly toxic hydroxyl free radical (HO ) via the Fenton reaction

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Lung lysates were measured spectrophotometrically using the Thiobarbituric Acid Reactive Substances (TBARS) assay kit procured from Cayman Chemicals (Cat # 10009055) following the manufacturers protocol (Cayman Chemicals, Ann Arbor, MI, United States)

Regions such as the hippocampus and posterior cingulate cortex exhibit early declines in mitochondrial efficiency and energy availability, contributing to synaptic failure long before widespread neuronal loss occurs