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Cells were then incubated at 37 C for the indicated time points in a mixture containing 1x Hanks balanced salt solution (HBSS, Gibco) or DMEM, each supplemented with 1 mM MgCl 2 , and 125 U Benzonase nuclease (Sigma/Pierce)

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This correlation significantly impacts the proper orchestration of signaling, leading to profound changes in immune response, cell differentiation, and functionality (15)