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NOR-5-treated schistosomula were cultivated in SCM, but for those exposed to SIN-1, the medium was changed to M199 (Thermo Fisher Scientific) supplemented by 0.1% (w/v) glucose, 1% (w/v) BSA and antibiotic-antimycotic solution (100 U/ml penicillin, 0.1 mg/ml streptomycin, 0.25 g/ml amphotericin B
The pathophysiology of both FGIDs is based on a combination of motility disturbances, visceral hypersensitivity, low grade mucosal immune activation, and altered processing of gut-brain-signals
This is caused by things like physical stress, mental stress, NSAIDs, alcohol, pesticides and many other contributing factors