Briefly, with the participants still lying on the bench in a semi-recumbent position following the biopsy collection, 15 ml of blood was sampled from an antecubital vein via a 20-gage Venflon and was immediately analyzed to assess the Hb concentration using the HemoCue Hb 201+ System (HemoCue AB, ngelholm, Sweden) and the Hematocrit (Hct) concentration in quadruplet via the micro-method (3 min at 13,500 rpm)
For high-throughput sequencing, the GSE89632 (GPL14951) dataset, which includes 39 disease samples and 24 normal samples, was selected for analysis
References Novo Nordisk presents phase 3 data for next-generation amylin cagrilintide, leading to advancement into dedicated clinical Programme, 16 September 2025, Novo Nordisk, DAscanio AM, Mullally JA, Frishman WH
How close are miRNAs from clinical practice