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J Clin Oncol 27:26772685 Green DM, Sklar CA, Boice Jr JD, Mulvihill JJ, Whitton JA, Stovall M, Yasui Y (2009b) Ovarian failure and reproductive outcomes after childhood cancer treatment: results from the Childhood Cancer Survivor Study

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Cryopreserved PBMCs were thawed, the viability was measured postthaw using AO/PI viability dye on a Nexelcom Cellometer (Nexelcom, Lawrence, MA, United States), and then labeled with carboxyfluorescein succinimidyl ester (CFSE, eBioscience, San Diego, CA, United States)