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Junction PCR confirmed the knock-in event in the liver, and digital PCR indicated an efficiency of approximately 1% for a single administration of Donor+Cas9, consistent with previously reported in vivo knock-in efficiencies (Fig

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1 Introduction Cancer remains a leading cause of morbidity and mortality worldwide, affecting millions of individuals each year ( Vitamins and minerals, essential micronutrients required for numerous physiological functions, play a critical role in maintaining health and preventing diseases ( Much evidence is available in the literature regarding the potential protective effect of micronutrients in reducing cancer risk