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Type III PRMTs are currently the only known PRMT7, which produces only monomethylarginine
GST tagged fusion proteins bind to glutathione agarose and, after washing, are eluted with elution buffer with excess glutathione Reducing agents are common additions to protein purification buffers because they help keep many proteins from forming unnatural disulfide bonds and aggregating
Cells were scraped out with a pipette tip and transferred to a 1.5 ml microtube, boiled for 10 min at 95 C, vortexed at full speed for 510 s, and spinned-down before split into single-use aliquots kept at 30 C
As a result of the BCAT reaction, three different -keto acids are produced and are oxidized using a common branched-chain -keto acid dehydrogenase (BCKD) complex, yielding the three different CoA derivatives
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