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The PCR program included a 10 min denaturation step at 95 C, followed by 40 cycles of 15 s of denaturation at 95 C and 30 s of primer hybridization at 52 C and 30 s of polymerization at 60 C

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Addition of R-lipoic acid increases ATP synthesis and aortic blood flow during reoxygenation after hypoxia while S-lipoic acid had no effect on ATP synthesis and improved blood flow at only 10-fold the effective dose of R-lipoic