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Antibiotics like azithromycin, doxycycline etc are used in pulse or mini pulse therapy for control of acne flare ups

For Safranin O/Fast Green staining, slides were stained with hematoxylin (Mayer, Sigma) for 5 min, 0.005% Fast Green (Thermo Fisher Scientific, Pittsburgh, PA, United States) for 1 min, rinsed with 1% acetic acid, and stained with 0.1% Safranin O (Millipore, Billerica, MA, United States) for 20 min

doi: 10.3389/fendo.2018.00248 20 ReinstatlerLQiYPWilliamsonRS

Some patients reduce to a lower maintenance dose (1.0 to 1.7 mg weekly) after reaching their goal weight, while others continue at the full 2.4 mg dose