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Cell pellets were resuspended in 50 mM Tris-HCl, pH 8.0 and cells disrupted by sonication (SoniPrep Ultrasonic Disintegrator, Sanyo) with incubations on ice between sonication bursts

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To date, biochemical evidence for the proposed conversion of L-carnitine via MSA and D-malate into pyruvate and CO 2 is still pending