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The cells were then washed 3 times with PBS, the nuclei stained with DAPI (1:1,000, Molecular probes) in the PBS wash (5 min)

Therefore, we could have chosen any percentage within the 40% to 100% range to represent the average estradiol in females

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In calves experimentally coinfected with BRSV and Pasteurella multocida , feeding with Saccharomyces cerevisiae fermentation products (SCFP) attenuated pulmonary inflammatory responses and enhanced pulmonary plasminogen expression as well as the expression of genes involved in glutathione metabolism, indicating that gut-derived interventions can reshape systemic and pulmonary immunity 177