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Neurochemical parameters determination Calorimetric assay kits (Biodiagnostic Co., Egypt) were used to measure malondialdehyde (MDA), hydrogen peroxide (H 2 O 2 ), nitric oxide (NO), and glutathione reduced (GSH) in brain cortical tissue from all groups

Right gel shows a lot of GST-fusion protein in the flow-through indicating that insufficient glutathione agarose resin was used for this purification