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The ratio of postsynaptic mRFP- -actin labeled spines juxtaposed to Synapsin or VGLUTl spines was not different from control treated neurons suggesting treatment-induced spines are forming functional synapses (Figure 9A-D)

This minimizes particulate and microbial contamination and mirrors pharmaceutical cleanroom procedures

A Cardiorespiratory Digital Twin can ingest wearable data (heart-rate variability, sleep architecture, training load), biomarker data (inflammatory markers, MOTS-c levels, collagen-turnover markers), and genomic data (connective-tissue gene variants, mitochondrial DNA haplogroup) to create a continuously updated model of an individual athlete's physiological state

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